Abstract: Objective To establish glutaredoxin 3（GLRX3）-knocked down nasopharyngeal carcinoma（NPC） cell lines and explore the biological functions of the GLRX3 gene. Methods Retroviral vectors expressing shRNA against GLRX3 were constructed and transfected into HONE1 cells， and stable cell lines showing knocked-down GLRX3 expression were selected. Reduced GLRX3 tran-scription in the cell lines was confirmed by real-time quantitative PCR.The cells were examined in colony formation and wound heal-ing assays to explore the influence of GLRX3 on these processes. Results Four retroviral shRNA vectors targeting GLRX3（shRNA-pBINNS2） were constructed and stably transfected into HONE1 cells.GLRX3 transcription was effectively suppressed in the stable cell lines.Knocking down GLRX3 inhibited clonogenicity and migration. Conclusions Knocking down GLRX3 inhibits the malignant behavior of NPC cells， suggesting that GLRX3 is a candidate oncogene in NPC.

Key words: Nasopharyngeal neoplasms, Glutaredoxin 3, RNAi, shRNA, Retrovirus vector